Article
Duplicate polyphenol oxidase genes on barley chromosome 2H and their functional differentiation in the phenol reaction of spikes and grains.
Journal of experimental botany - 1 Sept 2010
Taketa Shin, Matsuki Kanako, Amano Satoko, Saisho Daisuke, Himi Eiko, Shitsukawa Naoki, Yuo Takahisa, Noda Kazuhiko, Takeda Kazuyoshi
Abstract excerpt
Polyphenol oxidases (PPOs) are copper-containing metalloenzymes encoded in the nucleus and transported into the plastids. Reportedly, PPOs cause time-dependent discoloration (browning) of end-products of wheat and barley, which impairs their appearance quality. For this study, two barley PPO homologues were amplified using PCR with a primer pair designed in the copper binding domains of the wheat PPO genes. The...
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