Article
Comparison of mitochondrial DNA sequences of seven morphospecies of black flies (Diptera: Simuliidae).
Genome - 1 Apr 1991
Xiong B, Kocher T D
Abstract excerpt
Universal primers constructed from the 16S ribosomal RNA gene in the Drosophila yakuba mitochondrial genome were successfully used to amplify, via the polymerase chain reaction, the homologous region of mitochondrial DNA from seven black fly morphospecies. Amplification was achieved from single larval salivary glands and from single adults preserved in Carnoy's fixative (ethanol - acetic acid, 3:1), allowing DNA...
Topics
- Animals
- Base Composition
- Base Sequence
- DNA, Mitochondrial
- Electrophoresis, Polyacrylamide Gel
- Genetic Variation
- Molecular Sequence Data
- Nucleic Acid Conformation
- Phylogeny
- Polymerase Chain Reaction
- RNA, Ribosomal, 16S
