Article
Quantitative multiplex real-time PCR for detecting class 1, 2 and 3 integrons
11 Jun 2010
Abstract excerpt
OBJECTIVES: Integrons are bacterial genetic elements that can capture and express genes contained in mobile cassettes. Integrons have been described worldwide in Gram-negative bacteria and are a marker of antibiotic resistance. We developed a specific and sensitive Taqman probe-based real-time PCR method with three different primer-probe pairs for simultaneous detection of the three main classes of integron....
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