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Article

A novel multiplex PCR method for Clostridium botulinum neurotoxin type A gene cluster typing.

Microbiology and immunology - 1 May 2010

Umeda Kaoru, Seto Yoshiyuki, Kohda Tomoko, Mukamoto Masafumi, Kozaki Shunji

Abstract excerpt

A rapid, simple and sensitive multiplex PCR method for boNT/A gene cluster typing was developed by combining the results of BoNT/A subtype (boNT/A1 or /A2) gene detection with ha33 and/or p47 gene detection. Ten isolates associated with infant botulism in Japan were examined and divided into boNT...

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PMID: 20536728
DOI: 10.1111/j.1348-0421.2010.00213.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-20536728-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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