Article
Development of a species-diagnostic marker and its application for population genetics studies of the stingless bee Trigona collina in Thailand.
Genetics and molecular research : GMR - 18 May 2010
Theeraapisakkun M, Klinbunga S, Sittipraneed S
Abstract excerpt
A molecular maker for authenticating species origin of the stingless bee (Trigona collina) was developed. Initially, amplified fragment length polymorphism analysis was made of 11 stingless bee species using 64 primer combinations. A 316-bp band found only in T. collina was cloned and sequenced. A primer pair (CUTc1-F/R) was designed and tested for species-specificity in 15 stingless bee species (239 nests). The...
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