Article
Efficiency of RAPD and ISSR markers in differentiation of homo- and heterokaryotic protoclones of Agaricus bisporus.
Journal of microbiology and biotechnology - 1 Apr 2010
Mahmudul Islam Nazrul, Bian Yin-Bing
Abstract excerpt
Morphologically, nine different slow-growing protoclones were screened from regenerated protoplast of heterokaryotic Agaricus bisporus. The present study is the first report of fingerprinting on differentiating homo- and heterokaryotic protoclones using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. Among 80 primers tested, the seven ISSR and seven RAPD primers selected...
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