Article
Characteristics of an extensive Mycobacterium avium subspecies paratuberculosis recombinant protein set.
Protein expression and purification - 1 Aug 2010
Bannantine John P, Stabel Judith R, Bayles Darrell O, Geisbrecht Brian V
Abstract excerpt
In the first step of a comprehensive large-scale antigen discovery project, 651 genes of Mycobacterium avium subspecies paratuberculosis were expressed in Escherichia coli. All of these were purified by affinity chromatography, dialyzed in phosphate buffered saline, and analyzed on SDS-PAGE gels....
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