Article
SNP discovery and genetic mapping of T-DNA insertional mutants in Fragaria vesca L.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik - 1 Aug 2010
Ruiz-Rojas J J, Sargent D J, Shulaev V, Dickerman A W, Pattison J, Holt S H, Ciordia A, Veilleux Richard E
Abstract excerpt
As part of a program to develop forward and reverse genetics platforms in the diploid strawberry [Fragaria vesca L.; (2n = 2x = 14)] we have generated insertional mutant lines by T-DNA mutagenesis using pCAMBIA vectors. To characterize the T-DNA insertion sites of a population of 108 unique single copy mutants, we utilized thermal asymmetric interlaced PCR (hiTAIL-PCR) to amplify the flanking region surrounding...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.