Article
Direct culture-independent Strain typing of Burkholderia cepacia complex in sputum samples from patients with cystic fibrosis.
Journal of clinical microbiology - 1 May 2010
Drevinek Pavel, Vosahlikova Sarka, Dedeckova Klara, Cinek Ondrej, Mahenthiralingam Eshwar
Abstract excerpt
We examined if multilocus sequence typing (MLST), a method for genotyping and species identification of Burkholderia cepacia complex bacteria, could be applied directly to cystic fibrosis sputum. The redesigned nested-PCR MLST format was successfully used to accurately identify strains in 23 sput...
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