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Article

High-throughput genome sequencing of two Listeria monocytogenes clinical isolates during a large foodborne outbreak.

BMC genomics - 18 Feb 2010

Gilmour Matthew W, Graham Morag, Van Domselaar Gary, Tyler Shaun, Kent Heather, Trout-Yakel Keri M, Larios Oscar, Allen Vanessa, Lee Barbara, Nadon Celine

Abstract excerpt

BACKGROUND: A large, multi-province outbreak of listeriosis associated with ready-to-eat meat products contaminated with Listeria monocytogenes serotype 1/2a occurred in Canada in 2008. Subtyping of outbreak-associated isolates using pulsed-field gel electrophoresis (PFGE) revealed two similar but distinct AscI PFGE patterns. High-throughput pyrosequencing of two L. monocytogenes isolates was used to rapidly...

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Food Contamination
Foodborne Diseases
Genomic Islands
High-Throughput Screening Assays
Listeria monocytogenes

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PMID: 20167121
DOI: 10.1186/1471-2164-11-120
PMCID: PMC2834635

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