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Quantitative analysis of plant polyamines including thermospermine during growth and salinity stress.

Plant physiology and biochemistry : PPB - 1 Jul 2010

Naka Yukie, Watanabe Kanako, Sagor G H M, Niitsu Masaru, Pillai M Arumugam, Kusano Tomonobu, Takahashi Yoshihiro

Abstract excerpt

Arabidopsis thaliana was thought to contain two spermine synthase genes, ACAULIS 5 (ACL5) and SPMS. Recent investigations, however, revealed that the ACL5 gene encodes thermospermine synthase. In this study, we have established a simple method to separate two isomers of tetraamine, spermine and thermospermine, in extracts from plant tissues of less than 500 mg. Polyamines (PAs) extracted from plant tissues were...

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PMID: 20137962
DOI: 10.1016/j.plaphy.2010.01.013

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