Article
Full length sequencing of all nine subtypes of the neuraminidase gene of influenza A viruses using subtype specific primer sets.
Journal of virological methods - 1 Apr 2010
Chander Yogesh, Jindal Naresh, Stallknecht David E, Sreevatsan Srinand, Goyal Sagar M
Abstract excerpt
An RT-PCR based method was developed using subtype specific overlapping primers to obtain full length amplification of neuraminidase (NA) gene from all subtypes (N1-N9) of influenza A viruses. This method was validated using reference strains of avian influenza viruses (AIV) (N1-N9), human influenza viruses (N1 and N2), and swine influenza viruses (N1-N3). Amplification of the NA gene was obtained with all...
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