Evaluation of multiplex type-specific real-time PCR assays using the LightCycler and joint biological agent identification and diagnostic system platforms for detection and quantitation of adult human respiratory adenoviruses.

Abstract excerpt

Every year, thousands of basic military trainees in each service of the U.S. Armed Forces experience acute respiratory disease. The majority of this disease burden results from infection with human adenoviruses. We designed single- and multiplex assays that detect and discriminate adenovirus types B3, E4, B7, B11, B14, and B21. A total of 116 oropharyngeal swab specimens obtained from patients at the Naval Health...

Topics

• Adenoviridae Infections
• Adenoviruses, Human
• Adult
• Genotype
• Humans
• Military Personnel
• Oropharynx
• Polymerase Chain Reaction
• Respiratory Tract Infections
• Sensitivity and Specificity