Article
A framework for the development of STR genotyping in domestic animal species: characterization and population study of 12 canine X-chromosome loci.
Electrophoresis - 1 Jan 2010
van Asch Barbara, Pinheiro Raquel, Pereira Rui, Alves Cíntia, Pereira Vânia, Pereira Filipe, Gusmão Leonor, Amorim António
Abstract excerpt
This study reports the methodology used to search, select and characterize STR loci on the canine X chromosome using publicly available genome resources and following the current guidelines for human and non-human forensic testing. After several rounds of selection, 12 X-STR markers were optimized for simultaneous co-amplification in a single PCR, and genetic profiles were determined in a sample of 103 unrelated...
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