Article
Development of multiplex real-time PCR assays for identification of members of the Anopheles funestus species group.
Malaria journal - 9 Dec 2009
Vezenegho Samuel B, Bass Chris, Puinean Mirel, Williamson Martin S, Field Linda M, Coetzee Maureen, Koekemoer Lizette L
Abstract excerpt
BACKGROUND: The malaria vector and non-vector species of the Anopheles funestus group are morphologically very similar and accurate identification is required as part of effective control strategies. In the past, this has relied on morphological and cytogenetic methods but these have been largely superseded by a robust allele-specific PCR (AS-PCR). One disadvantage of AS-PCR is the requirement for post-PCR...
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