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A rapid, 2-well, multiplex real-time polymerase chain reaction assay for the detection of SCCmec types I to V in methicillin-resistant Staphylococcus aureus.

Diagnostic microbiology and infectious disease - 1 Dec 2009

Valvatne Håvard, Rijnders Michelle I A, Budimir Ana, Boumans Marie-Louise, de Neeling Albert J, Beisser Patrick S, Stobberingh Ellen E, Deurenberg Ruud H

Abstract excerpt

For us to assess the spread of methicillin-resistant Staphylococcus aureus (MRSA), typing of the staphylococcal cassette chromosome mec (SCCmec) is a valuable addition to existing typing methods, such as multilocus sequence typing (MLST). Traditional SCCmec typing assays, that is, that of Oliveira et al. and Ito et al., are polymerase chain reaction (PCR) based, requiring electrophoresis. We introduce a rapid,...

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Identifiers

PMID: 19781888
DOI: 10.1016/j.diagmicrobio.2009.08.006

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