Article
A new polymorphic locus, D7S411, isolated by cloning from preparative pulse-field gels is close to the mutation causing cystic fibrosis.
Genomics - 1 Jan 1990
Ramsay M, Wainwright B J, Farrall M, Estivill X, Sutherland H, Ho M F, Davies R, Halford S, Tata F, Wicking C
Abstract excerpt
The mutation causing cystic fibrosis (CF) has been localized to the DNA sequence of 700 kb bounded by the loci identified by the markers pMP6d-9 (D7S399) and pJ3.11 (D7S8). A 560-kb fragment obtained after SacII digestion of DNA from a cell line containing this region of human chromosome 7 in a mouse background was separated using pulse-field gel electrophoresis and isolated from the gel. The DNA was digested...
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