Article
Bronze-2 gene of maize: reconstruction of a wild-type allele and analysis of transcription and splicing.
The Plant cell - 1 Nov 1990
Nash J, Luehrsen K R, Walbot V
Abstract excerpt
The maize Bronze-2 (Bz2) gene, whose product acts late in the anthocyanin biosynthetic pathway, has been cloned and its transcript has been mapped. We have developed a general procedure for reconstructing wild-type alleles from transposable element-induced mutants. An existing transposon-containing clone, bz2::mu1 [McLaughlin, M., and Walbot, V. (1987). Genetics 117, 771-776], was modified by replacing the region...
Topics
- Alleles
- Base Sequence
- Cloning, Molecular
- DNA Transposable Elements
- Introns
- Molecular Sequence Data
- Plant Proteins
- Polymerase Chain Reaction
- RNA Splicing
- RNA, Messenger
- Recombinant Fusion Proteins
