Article
Cloning and characterization of Rhizobium meliloti loci required for symbiotic root nodule invasion.
Molecular plant-microbe interactions : MPMI - 1 Jan 2000
Hoying J B, Behm S M, Lang-Unnasch N
Abstract excerpt
An immunological assay of root nodule polypeptides was used to analyze the nodules induced by 25 symbiotically defective Rhizobium meliloti mutants. Differences in polypeptide accumulation in these nodules were used to divide the mutants into three subsets. One subset, containing two mutant strains, was further analyzed. Nodules induced by these mutant strains lack both infection threads and bacteria. The...
Topics
- Blotting, Western
- Cloning, Molecular
- DNA Transposable Elements
- DNA, Bacterial
- Electrophoresis, Polyacrylamide Gel
- Genetic Complementation Test
- Kinetics
- Mutation
- Nitrogen Fixation
- Plant Cells
- Plant Development