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Identification and characterization of a cytochrome b559 Synechocystis 6803 mutant spontaneously generated from DCMU-inhibited photoheterotrophical growth conditions.

Biochimica et biophysica acta - 1 Oct 2009

Chiu Yi-Fang, Lin Wen-Ching, Wu Chia-Ming, Chen Yung-Han, Hung Chung-Hsien, Ke Shyue-Chu, Chu Hsiu-An

Abstract excerpt

We identified a spontaneously generated mutant from Synechocystis sp. PCC6803 wild-type cells grown in BG-11 agar plates containing 5 mM Glu and 10 microM DCMU. This mutant carries an R7L mutation on the alpha-subunit of cyt b559 in photosystem II (PSII). In the recent 2.9 A PSII crystal structural model, the side chain of this arginine residue is in close contact with the heme propionates of cyt b559. We called...

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PMID: 19464256
DOI: 10.1016/j.bbabio.2009.05.007

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