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Article

DNA sequencing by Microseq kit targeting 16S rRNA gene for species level identification of mycobacteria.

The Indian journal of medical research - 1 Feb 2009

Therese K Lily, Bartell John, Deepa P, Mangaiyarkarasi S, Ward Diedra, Dajcs Joseph, Madhavan H N, Stroman David

Abstract excerpt

BACKGROUND & OBJECTIVES: Identification of mycobacteria to the species level is of therapeutic significance. Conventional methods are laborious and time consuming so we did 16S rRNA sequencing using a commercial MicroSeq sequencing kit, which includes DNA sequencing with software package for identification and phylogenetic analysis of clinical mycobacterial isolates. METHODS: A total of 47 mycobacteria were...

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PMID: 19293445

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