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Metagenomic Analyses of Viruses in Stool Samples from Children with Acute Flaccid Paralysis

12 Feb 2009

Abstract excerpt

We analyzed viral nucleic acids in stool samples collected from 35 South Asian children with nonpolio acute flaccid paralysis (AFP). Sequence-independent reverse transcription and PCR amplification of capsid-protected, nuclease-resistant viral nucleic acids were followed by DNA sequencing and sequence similarity searches. Limited Sanger sequencing (35 to 240 subclones per sample) identified an average of 1.4...

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PMID: 19211756
DOI: 10.1128/jvi.02301-08

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