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Article

Recombinant expression and affinity purification of a novel epididymal human sperm-binding protein, BSPH1

5 Dec 2008

Abstract excerpt

Mammalian sperm undergo a series of maturation steps before acquiring fertilization competence. Our previous work demonstrated the importance of binder of sperm (BSP) proteins in bovine sperm capacitation. Recent studies identified a BSP-homologous DNA sequence in the human genome (BSPH1) and mRNA expression in the epididymis. The aim of this study was to develop an efficient method to express and purify...

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PMID: 19091820
DOI: 10.1093/molehr/gan077

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