Article
JC viral DNA chip allows geographical localization of unidentified cadavers for rapid identification.
Forensic science international. Genetics - 1 Jan 2008
Ikegaya Hiroshi, Iwase Hirotaro, Saukko Pekka J, Akutsu Tomoko, Sakurada Koichi, Yoshino Mineo
Abstract excerpt
To narrow down the geographical origins of unidentified cadavers, a rapid and simple method to detect JC virus (JCV) genotypes using a DNA chip was developed. Fifty-four probes on a 3mm silicon DNA chip were designed to distinguish 12 JCV genotypes. The detection limit of the DNA chip was 0.001 pg/microL of template JCV DNA concentration, showing a sensitivity equal to that of the conventional method. The...
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