Article
Multiplex single-base extension typing to identify nuclear genes required for RNA editing in plant organelles.
Nucleic acids research - 1 Feb 2009
Takenaka Mizuki, Brennicke Axel
Abstract excerpt
We developed a multiplex single-base extension single-nucleotide polymorphism-typing procedure for screening large numbers of plants for mutations in mitochondrial RNA editing. The high sensitivity of the approach detects changes in the RNA editing status generated in total cellular cDNA from pooled RNA preparations of up to 50 green plants. The method has been employed to tag several nuclear encoded genes...
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