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Article

First Isolation of Besnoitia besnoiti from a Chronically Infected Cow in Spain

1 Apr 2009

Abstract excerpt

Besnoitia besnoiti was isolated from a skin biopsy of a chronically infected cow from central Spain. Zoites released from macroscopic cysts were adapted to its culture in vitro on a MARC-145 cell monolayer. Tachyzoites produced in vitro were either cryopreserved or used for genomic DNA isolation. A 2206 nt sequence containing 18S ribosomal RNA gene, internal transcribed spacer 1 (ITS 1), and a partial sequence of...

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PMID: 18803440
DOI: 10.1645/ge-1772.1

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