Article
[Molecular identification of heparin from pigs by allele-specific PCR (AS-PCR) and amplification refractory mutation system (ARMS)].
Yao xue xue bao = Acta pharmaceutica Sinica - 1 May 2008
Yu Zhi-Yong, Ding Ge, Ding Xiao-Yu, Chu Bi-Hai, Qian Liang, Gu Sun
Abstract excerpt
The aim of this study is to develop a convenient and effective method for the identification of heparin from pigs (include Sus scrofa domestica Brisson and Sus scrofa riukiuanus). Based on sequences of D-loop region of pigs and the other animals, two pairs of highly specific primers were designed for distinguishing heparin of pigs from other animals. The primers were employed to amplify D-loop region of DNA...
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