Article
Loop-mediated isothermal amplification for discriminating between human herpesvirus 6 A and B.
Journal of virological methods - 1 Dec 2008
Ihira Masaru, Ohta Akane, Sugata Ken, Suga Sadao, Asano Yoshizo, Yoshikawa Tetsushi
Abstract excerpt
Genotyping of human herpesvirus 6 (HHV-6) is important clinically, particularly for the diagnosis of neurological diseases. The objective of this study was to establish a rapid HHV-6 genotyping method using the loop-mediated isothermal amplification (LAMP) method. An AccI site is located in the target sequence of HHV-6 B, but not in HHV-6 A. LAMP products were digested with the AccI enzyme and then separated by...
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