Article
Multiplex PCRs for assignment of Staphylocoagulase types and subtypes of type VI Staphylocoagulase.
Journal of microbiological methods - 1 Oct 2008
Sakai Fumihiko, Takemoto Atsuhiro, Watanabe Shinya, Aoyama Kenji, Ohkubo Tatsuro, Yanahira Shuichi, Igarashi Hideo, Kozaki Shunji, Hiramatsu Keiichi, Ito Teruyo
Abstract excerpt
Staphylocoagulases (SCs) have been classified by the differences in antigenicity using a serological method. We have developed a system to classify them based on the nucleotide differences in SC genes (coa). The system was composed of three multiplex PCRs (M-PCRs): M-PCR:A, identifying types III, IV, VII, and VIII; M-PCR:B, identifying types I, II, V, and VI; M-PCR:C, identifying three subtypes of type VI. In...
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