Article
aPCR-SSCP and DNA sequencing detecting two silent SNPs at KAP8.1 gene in the cashmere goat.
Molecular biology reports - 1 Jul 2009
Zhao M, Chen H, Wang X, Yu H, Wang M, Wang J, Lan X Y, Zhang C F, Zhang L Z, Guo Y K, Zhang B, Hu S R
Abstract excerpt
Keratin-associated proteins 8.1 gene (KAP8.1) is a structural gene responsible for the cashmere. KAP8.1 protein contains high glycine and tyrosine, which concerns regulation and function of the matrix structure fiber. In this study, the polymorphism of KAP8.1 gene was detected by methods of aPCR-SSCP (asymmetric polymerase chain reaction single-strand conformation polymorphism) and DNA sequencing in 791...
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