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Article

Detection of somaclonal variation of cotton (Gossypium hirsutum) using cytogenetics, flow cytometry and molecular markers.

Plant cell reports - 1 Aug 2008

Jin Shuangxia, Mushke Ramesh, Zhu Huaguo, Tu Lili, Lin Zhongxu, Zhang Yanxin, Zhang Xianlong

Abstract excerpt

Two protocols of plant regeneration for cotton were adopted in this study, namely, 2, 4-D and kinetin hormone combination and IBA and kinetin hormone combination. Twenty-eight embryogenic cell lines via somatic embryogenesis and 67 regenerated plants from these embryogenic calli were selected and used for random amplified polymorphic DNA (RAPD), simple sequence repeat (SSR), chromosomal number counting, and flow...

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PMID: 18504587
DOI: 10.1007/s00299-008-0557-2

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