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Article

Trapping of the thioacylglyceraldehyde-3-phosphate dehydrogenase intermediate from Bacillus stearothermophilus. Direct evidence for a flip-flop mechanism.

The Journal of biological chemistry - 1 Aug 2008

Moniot Sébastien, Bruno Stefano, Vonrhein Clemens, Didierjean Claude, Boschi-Muller Sandrine, Vas Mária, Bricogne Gérard, Branlant Guy, Mozzarelli Andrea, Corbier Catherine

Abstract excerpt

The crystal structure of the thioacylenzyme intermediate of the phosphorylating glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from Bacillus stearothermophilus has been solved at 1.8A resolution. Formation of the intermediate was obtained by diffusion of the natural substrate within the crystal of the holoenzyme in the absence of inorganic phosphate. To define the soaking conditions suitable for the isolation...

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Identifiers

PMID: 18480053
DOI: 10.1074/jbc.M802286200

Genes

GAPDH

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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