Article
Identification of clinically relevant yeast species by DNA sequence analysis of the D2 variable region of the 25-28S rRNA gene.
Mycoses - 1 May 2008
Putignani Lorenza, Paglia Maria G, Bordi Eugenio, Nebuloso Elena, Pucillo Leopoldo P, Visca Paolo
Abstract excerpt
Clinically relevant yeasts are conventionally identified by a combination of phenotypic tests, which occasionally provide ambiguous results for atypical isolates or uncommon species. In this study, we evaluate a direct polymerase chain reaction-sequencing method, which exploits sequence divergence in the hypervariable D2 region of the large subunit of the 25-28S ribosomal RNA (rRNA) gene for identification of...
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