Endonuclease restriction of SCAR amplicons SC811 is required to identify Ns-false-positive markers in PVS-susceptible potato cultivars.

Abstract excerpt

The Ns gene confers resistance of potato to Potato virus S (PVS). Sixteen German and Dutch potato cultivars, all registered in Poland, were found to be susceptible to PVS infection. However, scoring of the cultivars for the presence of the Ns-linked SCAR marker SC811(454) revealed additional amplicons with a similar electrophoretic migration rate as that of SC811(454), which resulted in ambiguous determination of...

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