Article
[Optimized multiplex PCR sets and genetic polymorphism of 30 microsatellite loci in domestic buffalo].
Yi chuan = Hereditas - 1 Jan 2008
Zhang Yi, Sun Dong-Xiao, Yu Ying, Zhang Yuan
Abstract excerpt
Nine multiplex-PCR-sets, including four triplex and five duplex combinations, were developed from 30 domestic buffalo microsatellite markers based on electrophoresis analysis of PCR products in denaturing polyacrylamide gels and visualization by silver-staining. Further, genetic variability on these microsatellites was examined in Xinglong buffalo of Hainan Province. Twenty-six loci exhibited genetic variation...
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