Article
The ATPase SpoIIIE transports DNA across fused septal membranes during sporulation in Bacillus subtilis.
Cell - 28 Dec 2007
Burton Briana M, Marquis Kathleen A, Sullivan Nora L, Rapoport Tom A, Rudner David Z
Abstract excerpt
The FtsK/SpoIIIE family of ATP-dependent DNA transporters mediates proper chromosome segregation in dividing bacteria. In sporulating Bacillus subtilis cells, SpoIIIE translocates much of the circular chromosome from the mother cell into the forespore, but the molecular mechanism remains unclear. Using a new assay to monitor DNA transport, we demonstrate that the two arms of the chromosome are simultaneously...
Topics
- Bacillus subtilis
- Bacterial Proteins
- Cell Membrane
- Chromosome Segregation
- Chromosomes, Bacterial
- Conjugation, Genetic
- Cytokinesis
- DNA, Bacterial
- Fluorescence Recovery After Photobleaching
- Membrane Fusion
- Microscopy, Electron
- Microscopy, Fluorescence
- Models, Genetic