Article
The organization of high-affinity ammonium uptake in Arabidopsis roots depends on the spatial arrangement and biochemical properties of AMT1-type transporters.
The Plant cell - 1 Aug 2007
Yuan Lixing, Loqué Dominique, Kojima Soichi, Rauch Sabine, Ishiyama Keiki, Inoue Eri, Takahashi Hideki, von Wirén Nicolaus
Abstract excerpt
The AMMONIUM TRANSPORTER (AMT) family comprises six isoforms in Arabidopsis thaliana. Here, we describe the complete functional organization of root-expressed AMTs for high-affinity ammonium uptake. High-affinity influx of (15)N-labeled ammonium in two transposon-tagged amt1;2 lines was reduced by 18 to 26% compared with wild-type plants. Enrichment of the AMT1;2 protein in the plasma membrane and localization of...
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