Article
Polymorphisms in gyrA and gyrB genes among Mycobacterium avium subsp. paratuberculosis type I, II, and III isolates.
Journal of clinical microbiology - 1 Oct 2007
Castellanos E, Aranaz A, Romero B, de Juan L, Alvarez J, Bezos J, Rodríguez S, Stevenson K, Mateos A, Domínguez L
Abstract excerpt
The analysis of the gyrA and gyrB genes of a panel of Mycobacterium avium subsp. paratuberculosis isolates from types I, II, and III detected type-specific single nucleotide polymorphisms. Based on these results, we developed a PCR and restriction enzyme analysis to discriminate type I and III isolates. The application of this technique would be the unique strategy to characterize these strains when there is not...
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