Relative quantitation of virus population size in mixed genotype infections using sequencing chromatograms.

Abstract excerpt

In order to quantitatively distinguish between highly similar RNA sequences, specific primers or probes must be designed. Unfortunately, consistent and reliable results are not always obtained with conventional techniques. This study uses reverse transcription-PCR coupled with direct terminator sequencing to economically and efficiently distinguish between sequence types in pooled samples while providing accurate...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Base Sequence
• Share to Genotype
• Share to Plant Diseases