Article
Primary screening of blood donors by nat testing for HCV-RNA: development of an "in-house" method and results.
Revista do Instituto de Medicina Tropical de Sao Paulo - 1 Jan 2000
Wendel Silvano, Levi José Eduardo, Takaoka Deise Tihe, Silva Isabela Cristina, Castro Juliana Polachini de, Torezan-Filho Mário A, Ghaname Jorge, Gioachini Romualdo, Brandão Joselito, Durigon Edison Luis
Abstract excerpt
An "in-house" RT-PCR method was developed that allows the simultaneous detection of the RNA of the Hepatitis C Virus (HCV) and an artificial RNA employed as an external control. Samples were analyzed in pools of 6-12 donations, each donation included in two pools, one horizontal and one vertical, permitting the immediate identification of a reactive donation, obviating the need for pool dismembering. The whole...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
