Article
[Application of multiplex allele-specific PCR for authentication of Panax ginseng and P. quinquefolius].
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica - 1 Dec 2006
Cui Guang-hong, Tang Xiao-jing, Huang Lu-qi
Abstract excerpt
OBJECTIVE: Searching and identifying SNP in Panax species and using multiplex allele-specific PCR (MAS-PCR) to authenticate P. ginseng and P. quenquefolium. METHOD: Based on genbank database of Panax species, using DNAMAN to align the sequences, identify SNP of P. ginseng and P. quenquefolium. Design allele-specific primers for P. ginseng and P. quenquefolium, optimize the PCR reaction system including the usage...
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