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Article

Optimizing PCR Positivity Criterion for Detection of Herpes Simplex Virus DNA on Skin and Mucosa

1 Mar 2007

Abstract excerpt

In 1997, we developed a PCR assay for the detection of herpes simplex virus (HSV) DNA. Recently, we determined an optimal positivity criterion based on research specimens and a dilution study. We found that a cutoff of 50 HSV DNA copies/ml of swab specimen, a level 10-fold lower than our previous cutoff, minimizes misclassification.

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Identifiers

PMID: 17329447
DOI: 10.1128/jcm.01405-06

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Source receipt: pubmed, retrieved 7 Sept 2026.

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