Article
Functional proteome analysis of the banana plant (Musa spp.) using de novo sequence analysis of derivatized peptides.
Journal of proteome research - 1 Jan 2007
Samyn Bart, Sergeant Kjell, Carpentier Sebastien, Debyser Griet, Panis Bart, Swennen Rony, Van Beeumen Jozef
Abstract excerpt
We report the use of chemical derivatization with MALDI-MS/MS analysis for de novo sequence analysis. Using three frequently used homology-based search algorithms, we were able to identify more than 40 proteins from banana, a nonmodel plant with unsequenced genome. Furthermore, this approach allowed the identification of different isoforms. We also observed that the identification score obtained varied according...
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