Article
Flow cytometric analysis of within-strain variation in polysaccharide expression by Bacteroides fragilis by use of murine monoclonal antibodies.
Journal of medical microbiology - 1 Oct 1991
Lutton D A, Patrick S, Crockard A D, Stewart L D, Larkin M J, Dermott E, McNeill T A
Abstract excerpt
The reactivity of four different monoclonal antibodies (MAbs) with populations of Bacteroides fragilis NCTC 9343, enriched by density gradient centrifugation for a large capsule, small capsule and electron-dense layer (EDL) only visible by electronmicroscopy, was examined. The MAbs reacted strong...
Topics
- Animals
- Antibodies, Monoclonal
- Bacteroides fragilis
- Cross Reactions
- Epitopes
- Flow Cytometry
- Genetic Variation
- Immunoblotting
- Mice
- Mice, Inbred BALB C
- Microscopy, Electron
- Microscopy, Fluorescence
- Polysaccharides, Bacterial
- Species Specificity