Article
Detection of the common Hb F Sardinia [A gamma (E19)Ile----Thr] variant by isoelectric focusing in normal newborns and in adults affected by elevated fetal hemoglobin syndromes.
Clinica chimica acta; international journal of clinical chemistry - 15 May 1991
Masala B, Manca L
Abstract excerpt
A simple and rapid conventional isoelectric focusing technique for the detection of the silent Hb F Sardinia variant, containing the mutated A gamma T chain, is described. The method is based on thin-layer gels of shallow pH gradient (pH 6.7-7.7) and allows the direct detection of this rather com...
Topics
- Adult
- Chromatography, High Pressure Liquid
- Female
- Fetal Hemoglobin
- Hemoglobins, Abnormal
- Humans
- Infant, Newborn
- Isoelectric Focusing
- Italy
- Mutation
- Pregnancy
- Thalassemia