Article
Defective dystrophin in Duchenne and Becker dystrophy myotubes in cell culture.
Neurology - 1 Dec 1990
Sklar R M, Beggs A H, Lev A A, Specht L, Shapiro F, Brown R H
Abstract excerpt
We examined normal and dystrophic human myotubes in cell culture for expression of dystrophin, the protein product of the Duchenne muscular dystrophy locus. Dystrophin levels in developing myotubes detected by Western blotting increased after 24 hours and reached maximum levels after 10 days in fusion medium. We did not detect dystrophin in myotubes cultured from Duchenne myoblasts (7 cases). Myotubes from a...
Topics
- Adult
- Blotting, Northern
- Blotting, Southern
- Cells, Cultured
- Child
- Child, Preschool
- Chromosome Deletion
- DNA
- Dystrophin
- Exons
- Female
- Humans