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Fast and reliable PCR/sequencing/RFLP assay for identification of fungi in onychomycoses

16 Aug 2006

Abstract excerpt

Fusarium spp. and other non-dermatophyte fungi are repeatedly isolated from abnormal nails. To investigate whether these fungi are the aetiological agents of infection or simply transient contaminants, a PCR/sequencing/RFLP assay was developed for direct and routine identification of the infecting fungi in onychomycosis. Fungal DNA was readily extracted using a commercial kit after dissolving nail fragments in a...

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PMID: 16914650
DOI: 10.1099/jmm.0.46723-0

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