MULTILOCUS ENZYME ELECTROPHORESIS AND CYTOCHROME B GENE SEQUENCING–BASED IDENTIFICATION OF LEISHMANIA ISOLATES FROM DIFFERENT FOCI OF CUTANEOUS LEISHMANIASIS IN PAKISTAN

Abstract excerpt

Seventeen Leishmania stocks isolated from cutaneous lesions of Pakistani patients were studied by multilocus enzyme electrophoresis and by polymerase chain reaction amplification and sequencing of the cytochrome b (Cyt b) gene. Eleven stocks that expressed nine zymodemes were assigned to L. (Leishmania) major. All of them were isolated from patients in the lowlands of Larkana district and Sibi city in Sindh and...

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