Article
Developmental and hormonal regulation of direct shoot organogenesis and somatic embryogenesis in sugarcane (Saccharum spp. interspecific hybrids) leaf culture.
Plant cell reports - 1 Oct 2006
Lakshmanan Prakash, Geijskes R Jason, Wang Lifang, Elliott Adrian, Grof Christopher P L, Berding Nils, Smith Grant R
Abstract excerpt
Rapid and efficient in vitro regeneration methods that minimise somaclonal variation are critical for the genetic transformation and mass propagation of commercial varieties. Using a transverse thin cell layer culture system, we have identified some of the developmental and physiological constraints that limit high-frequency regeneration in sugarcane leaf tissue. Tissue polarity and consequently the orientation...
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