Article
[Effects of MTNR1A gene on litter size in a large white and a landrace herd].
Yi chuan = Hereditas - 1 Jul 2006
Wang Xiao-Feng, Wang Ai-Guo, Fu Jin-Lian, Li Zong-Jin, Han Li-Ping, Yang Shu-Ming
Abstract excerpt
Two pairs of primers were designed based on the known sequence in GenBank for amplification of MTNR1A gene in a Large White and a Landrace herd. Using PCR-SSCP (single strand conformation polymorphism), we found a single nucleotide polymorphism(SNP) within the product amplified from the first pair of primers. PCR products from randomly selected different genotypes were sequenced after were recovered and purified....
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